Mostafa, Mahmoud
Dr. Mahmoud Elhusseiny Mostafa joined the Center for Interdisciplinary Cardiovascular Sciences with over 16 years of experience across academic and industrial settings. His career shows a progressive focus on mass spectrometry, metabolomics, lipidomics, biochemistry, and molecular biology, with an emphasis on translational research.
He began his career in R&D and quality control, building a strong foundation in bacterial, human, and cell culturing techniques, analytical chemistry, chromatography, mass spectrometry, coding, and sequencing.
While earning his Ph.D. at Saint Louis University, Dr. Mostafa led the development of high-resolution metabolomics workflows combining supercritical fluid chromatography, nanospray ionization, and multi-plex isobaric tagging to analyze complex lipid classes and structural isomers. His work contributed to novel tools for quantifying and localizing unsaturated fatty acids and phospholipids. He advanced in lipidomics and fatty acid metabolism, employing stable isotope labeling and chemical tagging to dissect lipid signaling pathways and metabolic flux.
He also expanded his expertise to pathway analysis, disease biomarker discovery using targeted and untargeted omics, and confocal microscopy. He is proficient in coding and data analysis using R, Python, and Bash, with experience developing reproducible workflows, automating data pipelines, and performing advanced statistical and multivariate analyses on large-scale omics datasets.
His contributions have earned several national and international awards, including the Outstanding PhD Chemistry Student Award, Alpha Sigma Nu Honor Society Award, Dissertation Fellowship, Research Excellence Award (MSB 2023), Best Poster Award (MSB 2021), Top cited publication award from Wiley for 2021 and 2022, and an Analyst Journal Hot Article distinction (2023). He has presented at leading scientific meetings and authored multiple high-impact publications in analytical chemistry, lipidomics, and metabolomics.
In his first year at Harvard Medical School, Dr. Mostafa focused on multi-omics integration and advancing biochemical profiling to investigate molecular mechanisms underlying metabolic disease phenotypes. Dr. Mostafa joined CICS in 2025 to investigate ADP-ribosylated (ADPr) apolipoproteins, focusing on ADPr-APOA1-HDL, utilizing state-of-the-art mass spectrometry and imaging technologies.