Singh, Sasha A.
Assistant Professor of Medicine, Harvard Medical School;
Associate Scientist, Brigham and Women's Hospital;
Director of Proteomics Research, CICS
Dr. Sasha Singh earned her MSc in Developmental Genetics (2001) and PhD in Biochemistry/Protein Crystallography (2007) from the Department of Cell and Systems Biology at the University of Toronto (2007). Following her doctoral studies, she joined the labs of Steen and Steen at Children’s Hospital Boston and Harvard Medical School for her postdoctoral studies in mass spectrometry guided research. During this time, Dr. Singh contributed to several projects, including high-throughput proteomics screens and detailed investigations of protein post-translational modifications in relation to the cell cycle, cancer, and several neurological disorders. A highlight of her work during this time was the development of FLEXIQuant, a practical and effective workflow for implementing full-length protein mass spectrometry standards, which later evolved into a novel in vitro kinase assay known as FLEXIQinase.
Dr. Singh joined the Center for Interdisciplinary Cardiovascular Sciences in 2012 and has been an innovative leader ever since. She brings a diverse skill set, seamlessly integrating qualitative and quantitative mass spectrometry-guided experiments into the center’s research efforts. Her work has contributed significantly to the development of foundational research and target discovery platforms focused on understanding and tackling mechanisms of cardiovascular disease. Notably, Dr. Singh was the first to apply high-resolution, high-accuracy mass spectrometry to monitor protein turnover in humans. Her work has contributed to a paradigm shift in our understanding of “good HDL-cholesterol particle” metabolism, providing insight on why HDL-cholesterol-raising trials have failed to reduce major adverse cardiovascular events. Dr. Singh also actively investigates the effects of various post-translational modifications on protein function. Her lab has extensive expertise in developing bench-to-instrument workflows for ADP-ribosylation proteomics, advancing the study of this critical modification and its role in cellular processes.
Dr. Singh is a member of the American Society for Mass Spectrometry, the US Human Proteome Organization, the American Heart Association, and the International Society for Applied Cardiovascular Biology.
Publications
ShowSantinelli-Pestana DV, Delwarde C, Kasai T, Kuraoka S, Nakamura Y, Okada T, Decano JL, Chelvanambi S, Ge R, Mlynarchik AK, Perez K, Campedelli A, Sonawane AR, Aikawa E, Singh SA, Aikawa M
Clift CL, Blaser MC, Bartoli-Leonard F, Schlotter F, Higashi H, Kuraoka S, Kasai T, Turner ME, Pham T, Perez KA, Robson SC, Body SC, Muehlschlegel JD, Aikawa M, Singh SA, Aikawa E
Clift CL, Goudot G, Bogut G, Kasai T, van Kampen A, Negrao Pantaleao A, Messas E, Aikawa M, Singh SA, Levine RA, Pernot M, Aikawa E
Delwarde C, Matamalas JT, Chelvanambi S, Kasai T, Shlayen G, Santinelli-Pestana DV, Zamani M, Aikawa E, Aikawa M, Singh SA
Boeck M, Yagi H, Lundgren P, Pivodic A, Nilsson AK, Zeng Y, Chen CT, Kasai T, Lee D, Nian S, Hirst V, Neilsen K, Wang C, Lee J, Yu M, McCutcheon A, Singh SA, Aikawa M, Bazinet RP, Fu Z, Hellström A, Smith LE
Cahalane RME, Meuris B, Matamalas JT, Blaser MC, Billaud M, Kasai T, Hendrickx A, Goeminne LJE, Muehlschlegel J, Rega F, Aikawa M, McNamara L, Singh SA, Clift CL, Aikawa E
Lupieri A, Becker-Greene D, Le TD, Roschel MM, Jha PK, Sonawane AR, Mullick A, Tsimikas S, Kuraoka S, Singh SA, Aikawa M, Aikawa E
Higuita ML, Ajenu EO, Bolger-Chen M, Olverson G, Akbari Ghavimi SA, Lee SY, Kasai T, Michaud W, Chijioke C, Ruidas B, Pitti A, Minie N, Catricala J, Vincent D, Li S, D'Alessandro D, Aikawa E, Singh SA, Billaud M, Osho AA, Tessier SN, Rabi SA
Raju S, Turner ME, Cao C, Abdul-Samad M, Punwasi N, Blaser MC, Cahalane RME, Botts SR, Prajapati K, Patel S, Wu R, Gustafson D, Galant NJ, Fiddes L, Chemaly M, Hedin U, Matic L, Seidman MA, Subasri V, Singh SA, Aikawa E, Fish JE, Howe KL
Jung B, Yagi H, Kuo A, Dorweiler TF, Aikawa M, Kasai T, Singh SA, Dannenberg AJ, Fu Z, Niaudet C, Smith LEH, Hla T
Bortolin RH, de Souza Leite F, Luchessi AD, Esposito J, Barbosa IN, Costa de Freitas RC, Sonawane AR, Singh SA, Aikawa E, Silva KAT, Hirata TDC, Neta APR, Goulart E, Caires-Júnior LC, Mata Martins TMD, Semedo P, de Paula Moreira D, Naslavsky M, Faludi AA, Gonçalves RM, Araujo DB, Malaquias VB, Ferreira GM, DeOcesano-Pereira C, Chudzinski-Tavassi AM, Pu WT, Zatz M, Hirata RDC, Hirata MH
Yagi H, Qiu C, Zeng Y, Boeck M, Nian S, Chen CT, Harman JC, Kasai T, Lee J, Hirst V, Neilsen K, Wang C, Bora K, Maurya M, Rodrick T, Grumbine M, Yang Y, Hua Z, Sweet IR, Singh SA, Aikawa M, Chen J, Fu Z
Boeck M, Yagi H, Chen CT, Zeng Y, Lee D, Nian S, Kasai T, Lee J, Hirst V, Wang C, Neilsen K, Rodrick TC, McCutcheon A, Yu M, Lodhi IJ, Singh SA, Aikawa M, Bazinet RP, Fu Z
Kasai T, Nakamura Y, Aikawa M, Singh SA
Boucher DM, Robichaud S, Lorant V, Leon JS, Suliman I, Rasheed A, Susser LI, Emerton C, Geoffrion M, De Jong E, Bowdish DME, Aikawa M, Aikawa E, Singh SA, Rayner KJ, Ouimet M
Halu A, Chelvanambi S, Decano JL, Matamalas JT, Whelan M, Asano T, Kalicharran N, Singh SA, Loscalzo J, Aikawa M
Costa de Freitas RC, Bortolin RH, Kuraoka S, Rogers MA, Blaser MC, Chelvanambi S, Borges JB, de Oliveira VF, Dagli-Hernandez C, Bastos GM, da Silva Rodrigues Marçal E, Malaquias VB, Gonçalves RM, Faludi AA, Silbiger VN, Luchessi AD, Aikawa M, Crespo Hirata RD, Singh SA, Aikawa E, Hirata MH
Yagi H, Boeck M, Nian S, Neilsen K, Wang C, Lee J, Zeng Y, Grumbine M, Sweet IR, Kasai T, Negishi K, Singh SA, Aikawa M, Hellström A, Smith LEH, Fu Z
Yagi H, Boeck M, Petrishka-Lozenska M, Lundgren P, Kasai T, Cagnone G, Neilsen K, Wang C, Lee J, Tomita Y, Singh SA, Joyal JS, Aikawa M, Negishi K, Fu Z, Hellström A, Smith LEH
Yagi H, Boeck M, Nian S, Neilsen K, Wang C, Lee J, Zeng Y, Grumbine M, Sweet IR, Kasai T, Negishi K, Singh SA, Aikawa M, Hellström A, Smith LEH, Fu Z
Okui T, Kuraoka S, Iwashita M, Itagawa R, Kasai T, Aikawa M, Singh SA, Aikawa E
Okui T, Kuraoka S, Iwashita M, Itagawa R, Kasai T, Aikawa M, Singh SA, Aikawa E
Yagi H, Boeck M, Petrishka-Lozenska M, Lundgren P, Kasai T, Cagnone G, gael.cagnone.1@gmail.com, Wang C, Lee J, Tomita Y, Singh SA, Joyal JS, Aikawa M, Negishi K, Fu Z, Hellström A, Smith LEH
Raju S, Turner ME, Cao C, Abdul-Samad M, Punwasi N, Blaser MC, Cahalane RM, Botts SR, Prajapati K, Patel S, Wu R, Gustafson D, Galant NJ, Fiddes L, Chemaly M, Hedin U, Matic L, Seidman M, Subasri V, Singh SA, Aikawa E, Fish JE, Howe KL
Reyes-Soffer G, Matveyenko A, Lignos J, Matienzo N, Santos Baez LS, Hernandez-One A, Yung L, Nandakumar R, Singh SA, Aikawa M, George R, Ginsberg HN
Aikawa E, Blaser MC, Singh SA, Levine RA, Yacoub MH
Cahalane RME, Turner ME, Clift CL, Blaser MC, Bogut G, Levy S, Kasai T, Driedonks TAP, Nolte-'t Hoen ENM, Aikawa M, Singh SA, Aikawa E
Clift CL, Blaser MC, Gerrits W, Turner ME, Sonawane A, Pham T, Andresen JL, Fenton OS, Grolman JM, Campedelli A, Buffolo F, Schoen FJ, Hjortnaes J, Muehlschlegel JD, Mooney DJ, Aikawa M, Singh SA, Langer R, Aikawa E
Shelbaya K, Arthur V, Yang Y, Dorbala P, Buckley L, Claggett B, Skali H, Dufresne L, Yang TY, Engert JC, Thanassoulis G, Floyd J, Austin TR, Bortnick A, Kizer J, Freitas RCC, Singh SA, Aikawa E, Hoogeveen RC, Ballantyne C, Yu B, Coresh J, Blaha MJ, Matsushita K, Shah AM
Raju S, Botts SR, Blaser MC, Abdul-Samad M, Prajapati K, Khosraviani N, Ho TWW, Breda LCD, Ching C, Galant NJ, Fiddes L, Wu R, Clift CL, Pham T, Lee WL, Singh SA, Aikawa E, Fish JE, Howe KL
Andraski AB, Sacks FM, Aikawa M, Singh SA
Tzani A, Haemmig S, Cheng HS, Perez-Cremades D, Heuschkel MA, Jamaiyar A, Singh S, Aikawa M, Yu P, Wang T, Ye S, Feinberg MW, Plutzky J
Rogers MA, Bartoli-Leonard F, Zheng KH, Small AM, Chen HY, Clift CL, Asano T, Kuraoka S, Blaser MC, Perez KA, Natarajan P, Yeang C, Stroes ESG, Tsimikas S, Engert JC, Thanassoulis G, O'Donnell CJ, Aikawa M, Singh SA, Aikawa E
Decano JL, Maiorino E, Matamalas JT, Chelvanambi S, Tiemeijer BM, Yanagihara Y, Mukai S, Jha PK, Pestana DVS, D'Souza E, Whelan M, Ge R, Asano T, Sharma A, Libby P, Singh SA, Aikawa E, Aikawa M
Kasai T, Kuraoka S, Higashi H, Delanghe B, Aikawa M, Singh SA
Perez KA, Deppe DW, Filas A, Singh SA, Aikawa E
Santinelli-Pestana DV, Aikawa E, Singh SA, Aikawa M
Blaser MC, Buffolo F, Halu A, Turner ME, Schlotter F, Higashi H, Pantano L, Clift CL, Saddic LA, Atkins SK, Rogers MA, Pham T, Vromman A, Shvartz E, Sukhova GK, Monticone S, Camussi G, Robson SC, Body SC, Muehlschlegel JD, Singh SA, Aikawa M, Aikawa E
Raju S, Botts SR, Blaser M, Prajapati K, Ho TWW, Ching C, Galant NJ, Fiddes L, Wu R, Clift CL, Pham T, Lee WL, Singh SA, Aikawa E, Fish JE, Howe KL
Andraski AB, Singh SA, Higashi H, Lee LH, Aikawa M, Sacks FM
Bartoli-Leonard F, Zimmer J, Sonawane AR, Perez K, Turner ME, Kuraoka S, Pham T, Li F, Aikawa M, Singh S, Brewster L, Aikawa E
Iqbal F, Schlotter F, Becker-Greene D, Lupieri A, Goettsch C, Hutcheson JD, Rogers MA, Itoh S, Halu A, Lee LH, Blaser MC, Mlynarchik AK, Hagita S, Kuraoka S, Chen HY, Engert JC, Passos LSA, Jha PK, Osborn EA, Jaffer FA, Body SC, Robson SC, Thanassoulis G, Aikawa M, Singh SA, Sonawane AR, Aikawa E
Katsuki S, Kumar Jha P, Lupieri A, Nakano T, Passos LSA, Rogers MA, Becker-Greene D, Le TD, Decano JL, Ho Lee L, Guimaraes GC, Abdelhamid I, Halu A, Muscoloni A, Vittorio Cannistraci C, Higashi H, Zhang H, Vromman A, Libby P, Keith Ozaki C, Sharma A, Singh SA, Aikawa E, Aikawa M
Kuo A, Checa A, Niaudet C, Jung B, Fu Z, Wheelock CE, Singh SA, Aikawa M, Smith LEH, Proia RL, Hla T
Morgan S, Lee LH, Halu A, Nicolau JS, Higashi H, Ha AH, Wen JR, Daugherty A, Libby P, Cameron SJ, Mix D, Aikawa E, Owens AP, Singh SA, Aikawa M
Atkins SK, Sonawane AR, Brouwhuis R, Barrientos J, Ha A, Rogers M, Tanaka T, Okui T, Kuraoka S, Singh SA, Aikawa M, Aikawa E
Atkins SK, Sonawane AR, Brouwhuis R, Barrientos J, Ha A, Rogers M, Tanaka T, Okui T, Kuraoka S, Singh SA, Aikawa M, Aikawa E
Dacano JL, Aikawa M, Singh SA
Decano JL, Iwamoto Y, Goto S, Lee JY, Matamalas JT, Halu A, Blaser M, Lee LH, Pieper B, Chelvanambi S, Silva-Nicolau J, Bartoli-Leonard F, Higashi H, Shibata H, Vyas P, Wang J, Gostjeva E, Body SC, Singh SA, Aikawa M, Aikawa E
Passos LSA, Jha PK, Becker-Greene D, Blaser MC, Romero D, Lupieri A, Sukhova GK, Libby P, Singh SA, Dutra WO, Aikawa M, Levine RA, Nunes MCP, Aikawa E
Sawada H, Katsumata Y, Higashi H, Zhang C, Li Y, Morgan S, Lee LH, Singh SA, Chen JZ, Franklin MK, Moorleghen JJ, Howatt DA, Rateri DL, Shen YH, LeMaire SA, Aikawa M, Majesky MW, Lu HS, Daugherty A
Rogers MA, Atkins SK, Zheng KH, Singh SA, Chelvanambi S, Pham TH, Kuraoka S, Stroes ESG, Aikawa M, Aikawa E
Tanaka T, Asano T, Okui T, Kuraoka S, Singh SA, Aikawa M, Aikawa E
Tanaka T, Asano T, Okui T, Kuraoka S, Singh SA, Aikawa M, Aikawa E
Iwata H, Osborn EA, Ughi GJ, Murakami K, Goettsch C, Hutcheson JD, Mauskapf A, Mattson PC, Libby P, Singh SA, Matamalas J, Aikawa E, Tearney GJ, Aikawa M, Jaffer FA
Rogers MA, Hutcheson JD, Okui T, Goettsch C, Singh SA, Halu A, Schlotter F, Higashi H, Wang L, Whelan MC, Mlynarchik AK, Daugherty A, Nomura M, Aikawa M, Aikawa E
Kuraoka S, Higashi H, Yanagihara Y, Sonawane AR, Mukai S, Mlynarchik AK, Whelan MC, Hottiger MO, Nasir W, Delanghe B, Aikawa M, Singh SA
Lupieri A, Nagata Y, Passos LSA, Beker-Greene D, Kirkwood KA, Wylie-Sears J, Alvandi Z, Higashi H, Hung JW, Singh SA, Bischoff J, Levine RA, Aikawa E
Hutcheson JD, Schlotter F, Creager MD, Li X, Pham T, Vyas P, Higashi H, Body SC, Aikawa M, Singh SA, Kos L, Aikawa E
Decano JL, Singh SA, Bueno CG, Lee LH, Halu A, Chelvanambi S, Matamalas JT, Zhang H, Mlynarchik AK, Qiao J, Sharma A, Mukai S, Wang J, Anderson DG, Ozaki CK, Libby P, Aikawa E, Aikawa M
Rogers MA, Hutcheson JD, Okui T, Goettsch C, Singh SA, Halu A, Schlotter F, Higashi H, Wang L, Whelan MC, Mlynarchik AK, Daugherty A, Nomura M, Aikawa M, Aikawa E
Atkins SK, Singh SA, Aikawa E
Okui T, Iwashita M, Rogers MA, Halu A, Atkins SK, Kuraoka S, Abdelhamid I, Higashi H, Ramsaroop A, Aikawa M, Singh SA, Aikawa E
Okui T, Iwashita M, Rogers MA, Halu A, Atkins SK, Kuraoka S, Abdelhamid I, Higashi H, Ramsaroop A, Aikawa M, Singh SA, Aikawa E
Singh SA, Andraski AB, Higashi H, Lee LH, Ramsaroop A, Sacks FM, Aikawa M
Schlotter F, de Freitas RCC, Rogers MA, Blaser MC, Wu PJ, Higashi H, Halu A, Iqbal F, Andraski AB, Rodia CN, Kuraoka S, Wen JR, Creager M, Pham T, Hutcheson JD, Body SC, Kohan AB, Sacks FM, Aikawa M, Singh SA, Aikawa E
Rogers MA, Buffolo F, Schlotter F, Atkins SK, Lee LH, Halu A, Blaser MC, Tsolaki E, Higashi H, Luther K, Daaboul G, Bouten CVC, Body SC, Singh SA, Bertazzo S, Libby P, Aikawa M, Aikawa E
Kawakami R, Katsuki S, Travers R, Romero DC, Becker-Greene D, Araujo Passos LS, Higashi H, Blaser MC, Sukhova GK, Buttigieg J, Kopriva D, Schmidt AM, Anderson DG, Singh SA, Cardoso L, Weinbaum S, Libby P, Aikawa M, Croce K, Aikawa E
Engelbrecht E, Levesque MV, He L, Vanlandewijck M, Nitzsche A, Niazi H, Kuo A, Singh SA, Aikawa M, Holton K, Proia RL, Kono M, Pu WT, Camerer E, Betsholtz C, Hla T
Fehr AR, Singh SA, Kerr CM, Mukai S, Higashi H, Aikawa M
Arima N, Sasaki Y, Lee LH, Zhang H, Figueiredo JL, Mlynarchik AK, Qiao J, Yamada I, Higashi H, Ha AH, Halu A, Mizuno K, Singh SA, Yamazaki Y, Aikawa M
Andraski AB, Singh SA, Lee LH, Higashi H, Smith N, Zhang B, Aikawa M, Sacks FM
Goto S, Rogers MA, Blaser MC, Higashi H, Lee LH, Schlotter F, Body SC, Aikawa M, Singh SA, Aikawa E
Higashi H, Maejima T, Lee LH, Yamazaki Y, Hottiger MO, Singh SA, Aikawa M
Higashi H, Maejima T, Lee LH, Yamazaki Y, Hottiger MO, Singh SA, Aikawa M
Creager MD, Hohl T, Hutcheson JD, Moss AJ, Schlotter F, Blaser MC, Park MA, Lee LH, Singh SA, Alcaide-Corral CJ, Tavares AAS, Newby DE, Kijewski MF, Aikawa M, Di Carli M, Dweck MR, Aikawa E
Lee LH, Halu A, Morgan S, Iwata H, Aikawa M, Singh SA
Halu A, Wang JG, Iwata H, Mojcher A, Abib AL, Singh SA, Aikawa M, Sharma A
Schlotter F, Halu A, Goto S, Blaser MC, Body SC, Lee LH, Higashi H, DeLaughter DM, Hutcheson JD, Vyas P, Pham T, Rogers MA, Sharma A, Seidman CE, Loscalzo J, Seidman JG, Aikawa M, Singh SA, Aikawa E
Singh SA, Aikawa M
Lee LH, Andraski AB, Pieper B, Higashi H, Sacks FM, Aikawa M, Singh SA
Iwata H, Goettsch C, Sharma A, Ricchiuto P, Goh WW, Halu A, Yamada I, Yoshida H, Hara T, Wei M, Inoue N, Fukuda D, Mojcher A, Mattson PC, Barabási AL, Boothby M, Aikawa E, Singh SA, Aikawa M
Singh SA, Aikawa E, Aikawa M
Ghiassian SD, Menche J, Chasman DI, Giulianini F, Wang R, Ricchiuto P, Aikawa M, Iwata H, Müller C, Zeller T, Sharma A, Wild P, Lackner K, Singh S, Ridker PM, Blankenberg S, Barabási AL, Loscalzo J
Goettsch C, Hutcheson JD, Aikawa M, Iwata H, Pham T, Nykjaer A, Kjolby M, Rogers M, Michel T, Shibasaki M, Hagita S, Kramann R, Rader DJ, Libby P, Singh SA, Aikawa E
Singh SA, Andraski AB, Pieper B, Goh W, Mendivil CO, Sacks FM, Aikawa M
Singh SA, Miyosawa K, Aikawa M
Ricchiuto P, Iwata H, Yabusaki K, Yamada I, Pieper B, Sharma A, Aikawa M, Singh SA
Miyosawa K, Watanabe Y, Murakami K, Murakami T, Shibata H, Iwashita M, Yamazaki H, Yamazaki K, Ohgiya T, Shibuya K, Mizuno K, Tanabe S, Singh SA, Aikawa M
Hutcheson JD, Goettsch C, Pham T, Iwashita M, Aikawa M, Singh SA, Aikawa E
Singh SA, Winter D, Kirchner M, Chauhan R, Ahmed S, Ozlu N, Tzur A, Steen JA, Steen H
Itou T, Maldonado N, Yamada I, Goettsch C, Matsumoto J, Aikawa M, Singh S, Aikawa E
Singh S, Kirchner M, Steen JA, Steen H
Zhang X, Abreu JG, Yokota C, Macdonald BT, Singh S, Coburn KL, Cheong SM, Zhang MM, Ye QZ, Hang HC, Steen H, He X
Singh SA, Winter D, Bilimoria PM, Bonni A, Steen H, Steen JA
Akten B, Kye MJ, Hao le T, Wertz MH, Singh S, Nie D, Huang J, Merianda TT, Twiss JL, Beattie CE, Steen JA, Sahin M
Singh S, Springer M, Steen J, Kirschner MW, Steen H
Wang Z, Dong G, Singh S, Steen H, Li J
Singh S, Stavrinides J, Christendat D, Guttman DS
Singh S, Christendat D
Bonvin J, Aponte RA, Marcantonio M, Singh S, Christendat D, Turnbull JL
Singh SA, Christendat D
Sun W, Singh S, Zhang R, Turnbull JL, Christendat D
Singh S, Korolev S, Koroleva O, Zarembinski T, Collart F, Joachimiak A, Christendat D
Hardtke CS, Ckurshumova W, Vidaurre DP, Singh SA, Stamatiou G, Tiwari SB, Hagen G, Guilfoyle TJ, Berleth T